Mariela Segura

Biographie

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Personnel de laboratoire 

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Thème

Immunologie

Sujets de recherche

• Caractérisation des interactions entre les bactéries encapsulées et les cellules immunitaires de l’hôte. Rôle de la capsule polysaccharidique (CPS) dans la modulation de la réponse immunitaire innée et adaptative. Développement des vaccins à base de sucres.
• Bactéries à l’étude : Streptococcus Groupe B (SGB) et Streptococcus suis
• Modèle animal : souris et porc

Publications choisies

(2018, 2017, 2016, et quelques choisies de 2015-2014)

1. Roy, D., D. Takamatsu, M. Okura, G. Goyette-Desjardins, M.R. Van Calsteren, A. Dumesnil, M. Gottschalk, and M Segura. Streptococcus suis sialyltransferase exhibits alpha2-6 activity in Group B Streptococcus. Frontiers in Microbiology, In press, 2018.
2. Gottschalk, M. and Segura. Streptococcosis. In: Diseases of Swine. 11^th edition, John Wiley & Sons, Inc. In press, 2018.
3. Segura, M., N. Fittipaldi, C. Calzas and M. Gottschalk. Critical Streptococcus suis virulence factors: are they all really critical? Trends in Microbiology 25: 585-599, 2017. Invited review.
4. Lemire, P., T. Galbas, J. Thibodeau, and Segura. Natural Killer cell functions during the innate immune response to pathogenic streptococci. Frontiers in Microbiology, 8: e1196, 2017.
5. Auger, J.P., A. Santinon, D. Roy, K. Mossman, J. Xu, Segura, and M. Gottschalk. Type I interferon induced by Streptococcus suis serotype 2 is strain-dependent and may be beneficial for host survival. Frontiers in Immunology, 8: e1039, 2017.
6. Valanparambil, R.M., M. Tam, P. Gros, J.P. Auger, Segura, P. Gros, A. Jardim, T.G. Geary, K. Ozato, and M. Stevenson. IRF-8 regulates expansion of myeloid-derived suppressor cells and Foxp3+ regulatory T cells and modulates Th2 immune responses to gastrointestinal nematode infection. Plos Pathogens, 13: e1006647 2017.
7. Martelet, L., S. Lacouture, G. Goyette-Desjardins, G. Beauchamp, C. Surprenant, M. Gottschalk, and Segura. Porcine dendritic cells as an in vitro model to assess the immunological behaviour of Streptococcus suis subunit vaccine formulations and the polarizing effect of adjuvants. Pathogens, 6: e13, 2017.
8. Calzas, C., M. Taillardet, I.S. Fourati, D. Roy, M. Gottschalk, H. Soudeyns, T. Defrance, and Segura. Evaluation of the immunomodulatory properties of Streptococcus suis and group B Streptococcus capsular polysaccharides on the humoral response. Pathogens, 6: e16, 2017.
9. Roy, D., T. Athey, J.P. Auger, G. Goyette-Desjardins, M.R. Van Calsteren, D. Takamatsu, M. Okura, S. Teatero, M. Alcorlo, J.A. Hermoso, Segura, M. Gottschalk and N. Fittipaldi. A single amino acid polymorphism in the glycosyltransferase CpsK defines four Streptococcus suis serotypes. Scientific Reports, 7: e4066, 2017.
10. Zheng, H., X. Qiu, D. Roy, Segura, P. Du, J. Xu, and M. Gottschalk. Genotyping and investigating capsular polysaccharide synthesis gene loci of non-serotypeable Streptococcus suis isolated from diseased pigs in Canada. Veterinary Research, 48: e10, 2017.
11. Segura, M, C. Calzas, D. Grenier and M. Gottschalk. Initial steps of the pathogenesis of the infection caused by Streptococcus suis: fighting against non-specific defenses. FEBS Letters. 590: 3772-3799, 2016. Invited review.
12. Lecours, M.P., C. Letendre, D. Clarke, P. Lemire, T. Galbas, M.O. Benoit-Biancamano, J. Thibodeau, M. Gottschalk, and Segura. Immune-responsiveness of CD4+ T cells during Streptococcus suis serotype 2 infection. Scientific Reports, 6: e38061, 2016.
13. Auger, J.P., N. Fittipaldi, M.O. Benoit-Biancamano, Segura, and M. Gottschalk. Virulence studies of different sequence types and geographical origins of Streptococcus suis serotype 2 in a mouse model of infection. Pathogens, 5: e48, 2016.
14. Roy, D., D. Grenier, Segura, A. Mathieu-Denoncourt, and M. Gottschalk. Recruitment of Factor H to the Streptococcus suis cell surface is multifactorial. Pathogens, 5: e47, 2016.
15. Vinogradov, E., G. Goyette-Desjardins, M. Okura, D. Takamatsu, M. Gottschalk, and Segura. Structure determination of Streptococcus suis serotype 9 capsular polysaccharide and assignment of functions of the cps locus genes involved in its biosynthesis. Carbohydrate Research, 433: 25-30, 2016.
16. Hathroubi, S., F. Beaudry, C. Provost, L. Martelet, Segura, C. Gagnon and M. Jacques. Impact of Actinobacillus pleuropneumoniae biofilm mode of growth on the lipid A structures and stimulation of immune cells. Innate Immunity, 22: 353-362, 2016.
17. Goyette-Desjardins, G., C. Calzas, T. Shiao, A. Neubauer, J. Kempker, R. Roy, M. Gottschalk, and Segura. Protection against Streptococcus suis serotype 2 infection using a capsular polysaccharide glycoconjugate vaccine. Infection and Immunity, 84: 2059-2075, 2016.
18. Van Calsteren, M.R., G. Goyette-Desjardins, F. Gagnon, M. Okura, D. Takamatsu, R. Roy, M. Gottschalk, and Segura. Explaining the serological characteristics of Streptococcus suis serotypes 1 and 1/2 from their capsular polysaccharide structure and biosynthesis. Journal of Biological Chemistry, 291: 8387-8398, 2016.
19. Auray, G., C. Lachance, Y. Wang, C. Gagnon, Segura, and M. Gottschalk. Transcriptional analysis of PRRSV-infected porcine dendritic cell response to Streptococcus suis infection reveals up-regulation of inflammatory-related genes expression. PLOS One, 11: e0156019, 2016.
20. Price, N., G. Goyette-Desjardins, H. Nothaft, E. Valguarnera, C. Szymanski, Segura, and M. Feldman. Glycoengineered outer membrane vesicles: A novel platform for bacterial vaccines. Scientific Reports, 6: e24931, 2016.
21. Teatero, S., P. Lemire, K. Dewar, J. Wasserscheid, C. Calzas, G. Mallo, A. Li, T. Athey, Segura, and N. Fittipaldi. Genomic recombination leading to decreased virulence of Group B Streptococcus in a mouse model of adult invasive disease. Pathogens, 5 : e54, 2016.
22. Clarke, D., C. Letendre, M.P. Lecours, P. Lemire, T. Galbas, J. Thibodeau, and Segura. Group B Streptococcus induces a robust IFN-g response by CD4+ T cells in an in vitro and in vivo model. Journal of Immunology Research, 2016: e5290604, 2016.
23. Aguiar, C., J. Therrien, P. Lemire, Segura, L. Smith, and C. Theoret. Differentiation of equine induced pluripotent stem cells into a keratinocyte lineage. Equine Veterinary Journal, 48: 338-345, 2016.
24. Segura, M. Streptococcus suis vaccines: candidate antigens and progress. Expert Review of Vaccines. 14: 1587-608, Invited review.
25. Goyette-Desjardins, G., R. Roy and Segura. Murine whole-blood opsonophagocytosis assay to evaluate protection by antibodies raised against encapsulated extracellular bacteria. In: Carbohydrate-Based Vaccines – Methods and Protocols Edition of « Methods in Molecular Biology » lab protocol series. B. Lepenies (Ed.) Springer, 2015.
26. Calzas, C., P. Lemire, G. Auray, V. Gerdts, M. Gottschalk, and Segura. Antibody response specific to the capsular polysaccharide is impaired in Streptococcus suis serotype 2-infected animals. Infection and Immunity, 83: 441-453, 2015.
27. Segura, M. et al. Latest developments on Streptococcus suis: an emerging zoonotic pathogen: part 2. Future Microbiology 9: 587-591, 2014.
28. Segura, M. et al. Latest developments on Streptococcus suis: an emerging zoonotic pathogen: part 1. Future Microbiology 9: 441–444, 2014.
29. Goyette-Desjardins, G., J.-P. Auger, J. Xu, Segura and M. Gottschalk. Streptococcus suis, an important pig pathogen and emerging zoonotic agent – An update on the worldwide distribution based on serotyping and sequence typing. Emerging Microbes & Infections 3: e45, 2014. Invited review.
30. Lemire, P. and Segura. The NOD2 receptor modulates cytokine response but does not alter the clinical outcome of Group B Streptococcus-infected mice. Receptors & Clinical Investigation 2: 29-32, 2014. Invited Research Highlight.
31. Valanparambil, R.*, Segura*, M. Tam, A. Jardim, T. G. Geary, and M. M. Stevenson. Production and analysis of immunomodulatory excretory-secretory products from the mouse gastrointestinal nematode Heligmosomoides polygyrus bakeri. Nature Protocols, 9: 2740-2754, 2014.